The Therkildsen Lab held an informal workshop with hands-on instruction on executing our low-coverage sequencing pipeline (Therkildsen and Palumbi 2017). Dr. Gemma Clucas (Postdoc at University of New Hampshire) joined us for the workshop, which was led by our very own Dr. Aryn Wilder. Thank you Gemma for visiting, and Aryn for leading!
The Therkildsen lab is collaborating with Dr. Anne-Marie Dion-Côté (Postdoc in Barbash Lab at Cornell) to characterize the genomic architecture underlying local adaptation in the Atlantic silverside. Dr. Dion-Côté is training Maria Akopyan (PhD student in our lab) on cytogenetic analysis to characterize chromosomal variation between populations of the Atlantic silverside. Maria has been busy in the lab preparing metaphase spreads from regenerating fin tissue of silversides. Check out the photos from the lab below, including a karyotype of an individual from the New York population!
Aryn just came back from Hannes Baumann's Lab at the University of Connecticut with silverside juveniles we had spawned and raised there. We have two sets of offspring, one from parents collected in Georgia and another from parents collected on Long Island, New York. Fish from these locations have vastly different intrinsic growth capacity and also show genetic differences in a host of other traits.
We will continue to raise these fish here at Cornell so we can do cytogenetic analysis (in collaboration with Anne-Marie Dion-Côté) to compare the karyotypes of fish from the two populations.